Article
FMNL3 FH2-actin structure gives insight into formin-mediated actin nucleation and elongation.
Nature structural & molecular biology - 1 Jan 2013
Thompson Morgan E, Heimsath Ernest G, Gauvin Timothy J, Higgs Henry N, Kull F Jon
Abstract excerpt
Formins are actin-assembly factors that act in a variety of actin-based processes. The conserved formin homology 2 (FH2) domain promotes filament nucleation and influences elongation through interaction with the barbed end. FMNL3 is a formin that induces assembly of filopodia but whose FH2 domain is a poor nucleator. The 3.4-Å structure of a mouse FMNL3 FH2 dimer in complex with tetramethylrhodamine-actin...
Topics
- Actins
- Animals
- Binding Sites
- Cell Line
- Crystallography, X-Ray
- Formins
- Humans
- Jurkat Cells
- Mice
- Models, Molecular
- Mutation
- Protein Binding