Restriction endonuclease-mediated real-time digestion-PCR for somatic mutation detection.

Abstract excerpt

PCR is a powerful platform for clinical and diagnostic applications, but challenges remain in detecting somatic mutations, as mutant cells are often mixed with more numerous wild-type cells at the tissue-sample sites. Here, we describe a novel method that couples PCR with restriction endonuclease digestion (designated real-time digestion-PCR, or RTD-PCR) in a one-step reaction tube for detecting somatic mutations...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Exons
• Share to Amplified Fragment Length Polymorphism Analysis