Article
Detection of rare mutant alleles by restriction endonuclease-mediated selective-PCR: assay design and optimization.
Clinical chemistry - 1 May 2000
Fuery C J, Impey H L, Roberts N J, Applegate T L, Ward R L, Hawkins N J, Sheehan C A, O'Grady R, Todd A V
Abstract excerpt
BACKGROUND: Restriction endonuclease-mediated selective (REMS)-PCR, allows detection of point mutations, deletions, and insertions. Reactions require concurrent activity of a restriction endonuclease (RE) and a DNA polymerase, both of which must be sufficiently thermostable to retain activity during thermocycling. The inclusion of the RE in REMS-PCR inhibits amplification of sequences containing the RE...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.