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Article

Genetic Encoding of N6-(((Trimethylsilyl)methoxy)carbonyl)-l-lysine for NMR Studies of Protein-Protein and Protein-Ligand Interactions.

Journal of the American Chemical Society - 20 Jan 2021

Abdelkader Elwy H, Qianzhu Haocheng, Tan Yi Jiun, Adams Luke A, Huber Thomas, Otting Gottfried

Abstract excerpt

Trimethylsilyl (TMS) groups present outstanding NMR probes of biological macromolecules as they produce intense singlets in 1H NMR spectra near 0 ppm, where few other proton resonances occur. We report a system for genetic encoding of N6-(((trimethylsilyl)methoxy)carbonyl)-l-lysine (TMSK) for site-specific incorporation into proteins. The system is based on pyrrolysyl-tRNA synthetase mutants, which deliver...

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PMID: 33399460
DOI: 10.1021/jacs.0c11971

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