Article
A host-cell-selected variant of influenza B virus with a single nucleotide substitution in HA affecting a potential glycosylation site was attenuated in virulence for volunteers.
Archives of virology - 1 Jan 1990
Oxford J S, Schild G C, Corcoran T, Newman R, Major D, Robertson J, Bootman J, Higgins P, al-Nakib W, Tyrrell D A
Abstract excerpt
An influenza B virus was passaged in man (virus A) and then in human embryo trachea (C) and into embryonated eggs (D) or directly into eggs (B). Virus A, B, and C had the same (cell-like) haemagglutinin phenotype on reaction with selected monoclonal antibodies while D had an "egg-like" phenotype. The viruses were administered at a dose of 1,000 TCD50 (for MDCK cells) by intranasal inoculation to groups of 27 or...
Topics
- Amino Acid Sequence
- Base Sequence
- Genetic Variation
- Glycosylation
- Hemagglutination Inhibition Tests
- Hemagglutinins, Viral
- Humans
- Influenza B virus
- Molecular Sequence Data
- RNA, Viral