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Article

Thymidylate synthetase gene as a quantitative mutation marker in Chinese hamster cells.

Mutation research - 1 Mar 1990

Li I C, Chang C C, Trosko J E

Abstract excerpt

A quantitative mutation marker for cultured mammalian cells is presented which uses a selective medium containing folinic acid, aminopterin and thymidine (the 'FAT' medium) to select for mutants deficient in thymidylate synthetase (TS) activity. Optimization of FAT medium was carried out using Chinese hamster V79 cell lines having 3 levels of TS activity. By manipulating the concentration of folinic acid in FAT...

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Identifiers

PMID: 2308599
DOI: 10.1016/0165-7992(90)90096-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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