Thymidylate synthetase gene as a quantitative mutation marker in Chinese hamster cells.

doi:10.1016/0165-7992(90)90096-3

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Article

Thymidylate synthetase gene as a quantitative mutation marker in Chinese hamster cells.

Mutation research - 1 Mar 1990

Li I C, Chang C C, Trosko J E

Abstract excerpt

A quantitative mutation marker for cultured mammalian cells is presented which uses a selective medium containing folinic acid, aminopterin and thymidine (the 'FAT' medium) to select for mutants deficient in thymidylate synthetase (TS) activity. Optimization of FAT medium was carried out using Ch...

Topics

Animals
Cells, Cultured
Culture Media
Ethyl Methanesulfonate
Gene Expression
Genetic Markers
Hypoxanthine
Hypoxanthines
Leucovorin
Mutation
Thymidine
Thymidylate Synthase
Time Factors
Ultraviolet Rays

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