Oligonucleotide delivery by nucleofection does not rescue the reduced proliferation phenotype of gene-edited cells.

Abstract excerpt

Gene editing using single-stranded oligonucleotides (ODNs) can be used to reverse or create a single base mutation in mammalian cells. This approach could be used to treat genetic diseases caused, at least in part, by a nucleotide substitution. The technique could also be used as a tool to establish single base polymorphisms at multiple sites and thus aid in creating cell lines that can be used to define the...

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