Article
Development of a heme sensor using fluorescently labeled heme oxygenase-1.
Analytical biochemistry - 1 Feb 2013
Koga Shinya, Yoshihara Shun, Bando Hiroki, Yamasaki Kazuki, Higashimoto Yuichiro, Noguchi Masato, Sueda Shinji, Komatsu Hideyuki, Sakamoto Hiroshi
Abstract excerpt
Free heme, the protein-unbound form of heme, has both toxic and regulatory effects on cells. To detect free heme at low concentrations, we developed a heme sensor using fluorescently labeled heme oxygenase-1 (HO-1), an enzyme that catalyzes oxidative heme degradation and has a high affinity for heme. The response of the heme sensor is based on the fluorescence quenching that occurs when heme binds to the enzyme....
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