Visualizing Mitochondrial Heme Flow through GAPDH to Targets in Living Cells and its Regulation by NO

Abstract excerpt

Iron protoporphyrin IX (heme) is an essential cofactor that is chaperoned in mammalian cells by GAPDH in a process regulated by NO. To gain further understanding we generated a tetra-Cys human GAPDH reporter construct (TC-hGAPDH) which after being expressed and labeled with fluorescent FlAsH reagent could indicate heme binding by fluorescence quenching. When purified or expressed in HEK293T mammalian cells, FlAsH-...

Topics

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• Cell Line
• Gene Knockdown Techniques
• Glyceraldehyde-3-Phosphate Dehydrogenases
• Heme
• Mice
• Mutation
• Nitric Oxide Synthase Type II
• Protein Binding

Identifiers and source

Literature Corpus work

50c30791-dbcd-5ae5-a575-3516877ac64f

DOI

10.1101/2024.01.10.575067