Article
Oxygen equilibrium and EPR studies on alpha1beta1 hemoglobin dimer.
Journal of biochemistry - 1 Nov 2004
Venkatesh Balan, Miyazaki Gentaro, Imai Kiyohiro, Morimoto Hideki, Hori Hiroshi
Abstract excerpt
We undertook this project to clarify whether hemoglobin (Hb) dimers have a high affinity for oxygen and cooperativity. For this, we prepared stable Hb dimers by introducing the mutation Trp-->Glu at beta37 using our Escherichia coli expression system at the alpha1beta2 interface of Hb, and analyz...
Topics
- Dimerization
- Electron Spin Resonance Spectroscopy
- Heme
- Hemoglobins
- Molecular Weight
- Mutation
- Oxygen
- Protein Structure, Quaternary
- Protein Subunits