Article
Chinese hamster ovary cells deficient in peroxisomes lack the nonspecific lipid transfer protein (sterol carrier protein 2).
The Journal of biological chemistry - 5 Mar 1990
van Heusden G P, Bos K, Raetz C R, Wirtz K W
Abstract excerpt
Chinese hamster ovary cells deficient in intact peroxisomes were compared with wild type cells for the presence of the nonspecific lipid transfer protein (nsL-TP; sterol carrier protein 2). With the immunoblotting technique using the affinity purified antibody against rat liver nsL-TP, this protein was shown to be present in the homogenates from wild type cells, but could not be detected in mutant cells. In...
Topics
- Animals
- Carrier Proteins
- Cell Fractionation
- Cell Line
- Centrifugation, Density Gradient
- Cricetinae
- Cricetulus
- Female
- Immunoblotting
- Microbodies
- Molecular Weight