Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis

doi:10.1128/mbio.00604-13

Article

Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis

5 Nov 2013

Abstract excerpt

UNLABELLED: We constructed a near-saturation transposon mutant library for Burkholderia thailandensis, a low-virulence surrogate for the causative agent of melioidosis (Burkholderia pseudomallei). A primary set of nearly 42,000 unique mutants (~7.5 mutants/gene) was generated using transposon Tn5 derivatives. The strains carry insertions in 87% of the predicted protein-coding genes of the organism, corresponding...

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