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Article

Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G.

PloS one - 1 Jan 2012

Uyttendaele Isabel, Lavens Delphine, Catteeuw Dominiek, Lemmens Irma, Bovijn Celia, Tavernier Jan, Peelman Frank

Abstract excerpt

The mammalian two-hybrid system MAPPIT allows the detection of protein-protein interactions in intact human cells. We developed a random mutagenesis screening strategy based on MAPPIT to detect mutations that disrupt the interaction of one protein with multiple protein interactors simultaneously....

Read the complete abstract on PubMed

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Share to APOBEC-3G Deaminase
Share to Cytidine Deaminase
Share to gag Gene Products, Human Immunodeficiency Virus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutant Proteins

Share to Protein Interaction Mapping

Share to Protein Multimerization

Share to Two-Hybrid System Techniques

Share to vif Gene Products, Human Immunodeficiency Virus

Source

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Identifiers

PMID: 22970171
DOI: 10.1371/journal.pone.0044143
PMCID: PMC3438196

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-22970171-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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