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Article

Identification of human butyrylcholinesterase organophosphate-resistant variants through a novel mammalian enzyme functional screen.

The Journal of pharmacology and experimental therapeutics - 1 Dec 2012

Zhang Jun, Chen Sigeng, Ralph Erik C, Dwyer Mary, Cashman John R

Abstract excerpt

Human butyrylcholinesterase (hBChE) is currently being developed as a detoxication enzyme for the catalytic hydrolysis or stoichiometric binding of organophosphates (OPs). Previously, rationally designed hBChE mutants (G117H and E197Q) were reported in the literature and showed the feasibility of engineering OP hydrolytic functional activity into hBChE. However, the OP hydrolysis rate for G117H is too low for...

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Identifiers

PMID: 22956723
DOI: 10.1124/jpet.112.198499
PMCID: PMC3500536

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