Article
Analysis of substrates of protein kinase C isoforms in human breast cells by the traceable kinase method.
Biochemistry - 11 Sept 2012
Chen Xiangyu, Zhao Xin, Abeyweera Thushara P, Rotenberg Susan A
Abstract excerpt
A previous report [Abeyweera, T. P., and Rotenberg, S. A. (2007) Biochemistry 46, 2364-2370] described the application of the traceable kinase method in identifying substrates of protein kinase Cα (PKC-α) in nontransformed human breast MCF-10A cells. Here, a nonradioactive variation of this method compared the phosphoprotein profiles of three traceable PKC isoforms (α, δ, and ζ) for the purpose of identifying...
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