Article
Structure of the RNA claw of the DNA packaging motor of bacteriophage Φ29.
Nucleic acids research - 1 Oct 2012
Harjes Elena, Kitamura Aya, Zhao Wei, Morais Marc C, Jardine Paul J, Grimes Shelley, Matsuo Hiroshi
Abstract excerpt
Bacteriophage DNA packaging motors translocate their genomic DNA into viral heads, compacting it to near-crystalline density. The Bacillus subtilis phage 29 has a unique ring of RNA (pRNA) that is an essential component of its motor, serving as a scaffold for the packaging ATPase. Previously, deletion of a three-base bulge (18-CCA-20) in the pRNA A-helix was shown to abolish packaging activity. Here, we solved...
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