Article
The proximate 5' and 3' ends of the 120-base viral RNA (pRNA) are crucial for the packaging of bacteriophage phi 29 DNA.
Virology - 15 May 1994
Zhang C, Lee C S, Guo P
Abstract excerpt
In vitro mutagenesis was performed to identify the DNA packaging domain of the 120-base pRNA essential and specific for DNA encapsidation by bacteriophage phi 29 of Bacillus subtilis. All deletions and mutations targeted the 5' and 3' ends of the pRNA. DNA templates of a control or mutant pRNAs used for in vitro transcription with T7 RNA polymerase were generated by PCR. Fourteen mutant pRNA molecules were...
Topics
- Bacillus Phages
- Base Sequence
- Capsid
- DNA, Viral
- Models, Genetic
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- RNA, Viral
- Sequence Deletion