Article
Recombinant methionyl bovine prolactin: loss of bioactivity after single amino acid deletions from putative helical regions.
Molecular endocrinology (Baltimore, Md.) - 1 Jul 1990
Luck D N, Gout P W, Kelsay K, Atkinson T, Beer C T, Smith M
Abstract excerpt
We have previously described a method for producing recombinant methionyl bovine PRL (Met-bPRL), which is as bioactive as the authentic hormone in the Nb2 cell lactogen bioassay; in contrast, a Met-bPRL variant lacking tyrosine 28 was essentially devoid of bioactivity. In the present study we have investigated this loss of bioactivity at the molecular level by determining the bioactivities of a number of Met-bPRL...
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