Recombinant methionyl bovine prolactin: loss of bioactivity after single amino acid deletions from putative helical regions.

doi:10.1210/mend-4-7-1011

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Article

Recombinant methionyl bovine prolactin: loss of bioactivity after single amino acid deletions from putative helical regions.

Molecular endocrinology (Baltimore, Md.) - 1 Jul 1990

Luck D N, Gout P W, Kelsay K, Atkinson T, Beer C T, Smith M

Abstract excerpt

We have previously described a method for producing recombinant methionyl bovine PRL (Met-bPRL), which is as bioactive as the authentic hormone in the Nb2 cell lactogen bioassay; in contrast, a Met-bPRL variant lacking tyrosine 28 was essentially devoid of bioactivity. In the present study we hav...

Topics

Amino Acid Sequence
Chromosome Deletion
Gene Expression
Genetic Variation
Molecular Sequence Data
Prolactin
Protein Conformation
Recombinant Proteins

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