Article
Characterization of recombinant human brain-derived neurotrophic factor variants.
Archives of biochemistry and biophysics - 15 Dec 1999
Sunasara K M, Cramer S M, Hauer C R, Rupp R G, Shoup V A
Abstract excerpt
The purpose of this work was the chemical characterization of variants of the recombinant human brain derived neurotrophic factor (rHu-BDNF), expressed in Escherichia coli. This paper also addresses the question of the in vitro activity of these variants. Chemical characterization of the variants employed peptide mapping using Glu-C protease and cyanogen bromide digestion on reduced and alkylated variants...
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