Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity.

Nature methods - 1 Sept 2013

Xiang Zheng, Ren Haiyan, Hu Ying S, Coin Irene, Wei Jing, Cang Hu, Wang Lei

Abstract excerpt

Natural proteins often rely on the disulfide bond to covalently link side chains. Here we genetically introduce a new type of covalent bond into proteins by enabling an unnatural amino acid to react with a proximal cysteine. We demonstrate the utility of this bond for enabling irreversible binding between an affibody and its protein substrate, capturing peptide-protein interactions in mammalian cells, and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acyl-tRNA Synthetases
Share to CRF Receptor, Type 1
Share to Cysteine Endopeptidases

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Fluorescence

Share to Phenylalanine

Share to Photons

Share to Protein Engineering

Share to Receptors, Corticotropin-Releasing Hormone

Source

Open in PubMed Open full text

Identifiers

PMID: 23913257
DOI: 10.1038/nmeth.2595
PMCID: PMC3882359

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity.PMID 23913257Read ↗

Affinity‐Directed Site‐Specific Protein Labeling and Its Application to Antibody‐Drug ConjugatesSemantic proximity Read ↗

Developing Covalent Protein Drugs via Proximity-Enabled Reactive Therapeutics.Semantic proximity Read ↗

An engineered aryl azide ligase for site-specific mapping of protein-protein interactions through photo-cross-linking.Semantic proximity Read ↗

In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.Semantic proximity Read ↗

Site-specific photocoupling of pBpa mutated scFv antibodies for use in affinity proteomics.Semantic proximity Read ↗

Oxidation-induced generation of a mild electrophile for proximity-enhanced protein-protein crosslinking.Semantic proximity Read ↗

Redox-based reagents for chemoselective methionine bioconjugation.Semantic proximity Read ↗

Control of a tyrosyl radical mediated protein cross-linking reaction by electrostatic interaction.Semantic proximity Read ↗

Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodiesSemantic proximity Read ↗

Antibodies with infinite affinity: origins and applications.Semantic proximity Read ↗

Engineered peptide ligases for cell signaling and bioconjugation.Semantic proximity Read ↗

Designing redox potential-controlled protein switches based on mutually exclusive proteins.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.