Detection of single base differences using biotinylated nucleotides with very long linker arms.

doi:10.1093/nar/20.18.4831

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Article

Detection of single base differences using biotinylated nucleotides with very long linker arms.

Nucleic acids research - 25 Sept 1992

Livak K J, Hobbs F W, Zagursky R J

Abstract excerpt

A simple primer extension method for detecting nucleotide differences is based on the substitution of mobility-shifting analogs for natural nucleotides (1). This technique can detect any single-base difference that might occur including previously unknown mutations or polymorphisms. Two technical limitations of the original procedure have now been addressed. First, switching to Thermococcus litoralis DNA...

Topics

Base Sequence
Biotin
Cytochrome P-450 CYP2D6
Cytochrome P-450 Enzyme System
Deoxyadenine Nucleotides
Deoxycytosine Nucleotides
Deoxyuracil Nucleotides
Genetic Carrier Screening
Humans
Indicators and Reagents
Introns

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mixed Function Oxygenases

Molecular Sequence Data

Mutation

Oligodeoxyribonucleotides

Polymerase Chain Reaction

Polymorphism, Genetic

Tay-Sachs Disease

Source

Open in PubMed Open full text

Identifiers

PMID: 1408798
DOI: 10.1093/nar/20.18.4831
PMCID: PMC334239

Genes

APOE

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1408798-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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