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Article

Detection of single base differences using biotinylated nucleotides with very long linker arms.

Nucleic acids research - 25 Sept 1992

Livak K J, Hobbs F W, Zagursky R J

Abstract excerpt

A simple primer extension method for detecting nucleotide differences is based on the substitution of mobility-shifting analogs for natural nucleotides (1). This technique can detect any single-base difference that might occur including previously unknown mutations or polymorphisms. Two technical limitations of the original procedure have now been addressed. First, switching to Thermococcus litoralis DNA...

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Identifiers

PMID: 1408798
DOI: 10.1093/nar/20.18.4831
PMCID: PMC334239

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APOE

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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