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Article

A genotyping strategy based on incorporation and cleavage of chemically modified nucleotides.

Proceedings of the National Academy of Sciences of the United States of America - 20 Aug 2002

Wolfe Jia Liu, Kawate Tomohiko, Sarracino David A, Zillmann Martin, Olson Jeffrey, Stanton Vincent P, Verdine Gregory L

Abstract excerpt

Aiming to facilitate the analysis of human genetic variations in the context of disease susceptibility and varied drug response, we have developed a genotyping method that entails incorporation of a chemically labile nucleotide by PCR followed by specific chemical cleavage of the resulting amplicon at the modified bases. The identity of the cleaved fragments determines the genotype of the DNA. This method, termed...

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Identifiers

PMID: 12169663
DOI: 10.1073/pnas.162346699
PMCID: PMC123212

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Source receipt: pubmed, retrieved 7 Sept 2026.

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