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Article

Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles.

Clinical chemistry - 1 Feb 1995

Appel E, Eisenberg S, Roitelman J

Abstract excerpt

We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.

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PMID: 7874769

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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