Article
Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles.
Clinical chemistry - 1 Feb 1995
Appel E, Eisenberg S, Roitelman J
Abstract excerpt
We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Verni...
Topics
- Alleles
- Amino Acid Sequence
- Apolipoproteins E
- Base Sequence
- DNA
- Deoxyribonucleases, Type II Site-Specific
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction