Article
High-throughput single-nucleotide polymorphism genotyping by fluorescent competitive allele-specific polymerase chain reaction (SNiPTag).
Analytical biochemistry - 15 Feb 2002
McClay Joseph L, Sugden Karen, Koch Helga G, Higuchi Susumi, Craig Ian W
Abstract excerpt
Single nucleotide polymorphisms (SNPs) are becoming widely recognized as the new currency for gene mapping as increasing numbers are discovered. Here we outline a method for their rapid analysis based on an allele-specific polymerase chain reaction (PCR) which employs a competitive approach, whereby both allele-specific primers are present in the same reaction and carry different fluorescent labels. This...
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