Article
Partially overlapping substrate specificities of staphylococcal group A sortases.
Proteomics - 1 Oct 2012
Sibbald Mark J J B, Yang Xiao-Mei, Tsompanidou Eleni, Qu Di, Hecker Michael, Becher Dörte, Buist Girbe, van Dijl Jan Maarten
Abstract excerpt
Sortases catalyze the covalent attachment of proteins with a C-terminal LPxTG motif to the cell walls of Gram-positive bacteria. Here, we show that deletion of the srtA genes of Staphylococcus aureus and Staphylococcus epidermidis resulted in the dislocation of several LPxTG proteins from the cell wall to the growth medium. Nevertheless, proteomics and Western blotting analyses revealed that substantial amounts...
Topics
- Aminoacyltransferases
- Bacterial Proteins
- Biofilms
- Cysteine Endopeptidases
- Electrophoresis, Polyacrylamide Gel
- Genetic Complementation Test
- Membrane Proteins
- Mutation
- Phylogeny
- Protein Transport
- Sequence Alignment
- Staphylococcus aureus
- Staphylococcus epidermidis