Article
ABO blood group genotyping by quenching probe method.
Molecular and cellular probes - 1 Oct 2012
Tsutsumi Hirofumi, Asano Masatake, Hagiwara Yoshiyuki, Nogami Hiroaki, Izawa Hikaru, Maruyama Sayaka, Komuro Toshinobu
Abstract excerpt
We developed a multiplex ABO genotyping method with quenching probes (Q-probe). In this method, it is possible to discriminate the mutations, not only frequently used positions 261 and 796 but also position 703 in a single PCR. Each probe was designed to have cytosine residue at 5' or 3' end and labeled with three different fluorescence dyes, enabling the triplex detections of these polymorphisms. All...
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