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Article

A new method for ABO genotyping using fluorescence melting curve analysis based on peptide nucleic acid probes.

Legal medicine (Tokyo, Japan) - 1 Sept 2015

Lee Kyungmyung, Park Hyun-Chul, An Sanghyun, Ahn Eu-Ree, Lee Yang-Han, Kim Mi-Jung, Lee Eun-Jung, Park Jae Sin, Jung Jin Wook, Lim Sikeun

Abstract excerpt

ABO genotyping has been routinely used to identify suspects or unknown remains in crime investigations. Probe-based fluorescence melting curve analysis (FMCA) is a powerful tool for mutation detection and is based on melting temperature shifts due to thermal denaturation. In the present study, we developed a new method for ABO genotyping using peptide nucleic acid (PNA) probe-based FMCA. This method allowed for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25913644
DOI: 10.1016/j.legalmed.2015.04.001

Genes

ABO

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