Article
Detection of five common variants of ABO gene by a triplex probe-based fluorescence-melting-curve-analysis.
Analytical biochemistry - 1 Jul 2022
Soejima Mikiko, Koda Yoshiro
Abstract excerpt
Current studies have suggested that the ABO blood group system is associated with several clinical conditions. For large-scale genotyping of ABO alleles, we developed a triplex fluorescence melting curve analysis (FMCA) to determine five single nucleotide variants (SNVs), c.261delG, c.796C>A, c.802G>A and c.803G>C and c.1061delC, responsible for common ABO phenotypes using dual-labeled self-quenched (TaqMan)...
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