Article
A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus.
Vox sanguinis - 1 Jan 1995
Olsson M L, Chester M A
Abstract excerpt
An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
