Article
Genetic analysis of a vital mammalian housekeeping locus using CHO cells that express a transfected mutant allele.
Somatic cell and molecular genetics - 1 Nov 1990
Diaz J J, Rhoads D D, Roufa D J
Abstract excerpt
We describe a novel approach for the isolation of null mutations in a vital Chinese hamster ovary (CHO) cell housekeeping gene. Our experimental strategy required introduction of an expressible DNA clone encoding a recessive emetine-resistance allele of ribosomal protein S14 into wild-type CHO ce...
Topics
- Amino Acid Sequence
- Animals
- Cell Line
- Cloning, Molecular
- Cricetinae
- Cricetulus
- Electrophoresis, Gel, Two-Dimensional
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA, Messenger
- Restriction Mapping
- Ribosomal Proteins
- Transcription, Genetic
- Transfection