Article
Binding of the bacteriophage T4 regA protein to mRNA targets: an initiator AUG is required.
Nucleic acids research - 11 Dec 1990
Unnithan S, Green L, Morrissey L, Binkley J, Singer B, Karam J, Gold L
Abstract excerpt
Bacteriophage T4 regA protein translationally represses the synthesis of a subset of early phage-induced proteins. The protein binds to the translation initiation site of at least two mRNAs and prevents formation of the initiation complex. We show here that the protein binds to the translation initiation sites of other regA-sensitive mRNAs. Analysis of mRNA binding by filtration and nuclease protection assays...
Topics
- Base Sequence
- Binding Sites
- Binding, Competitive
- Cloning, Molecular
- Codon
- Escherichia coli
- Gene Expression Regulation, Viral
- Genes, Viral
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis