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Article

Allelic exchange in Actinomyces oris with mCherry fluorescence counterselection.

Applied and environmental microbiology - 1 Sept 2010

Wu Chenggang, Ton-That Hung

Abstract excerpt

Described here is a method for facile generation of markerless gene deletion mutants of Actinomyces oris. Homologous integration of a nonreplicative vector carrying a gene exchange cassette into the bacterial chromosome was selected for by using mCherry fluorescence and resistance to kanamycin. Completion of allelic replacement was counterselected for by using loss of fluorescence.

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 20601506
DOI: 10.1128/AEM.00811-10
PMCID: PMC2935053

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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