Article
Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks.
Yeast (Chichester, England) - 1 Jan 1994
Larionov V, Kouprina N, Eldarov M, Perkins E, Porter G, Resnick M A
Abstract excerpt
Rearrangements within plasmid DNA are commonly observed during transformation of eukaryotic cells. One possible cause of rearrangements may be recombination between repeated sequences induced by some lesions in the plasmid. We have examined the mechanisms of transformation-associated recombination in the yeast Saccharomyces cerevisiae using a plasmid system which allowed the effects of physical state and/or...
Topics
- DNA Damage
- DNA Repair Enzymes
- DNA, Circular
- DNA, Fungal
- DNA-Binding Proteins
- Endonucleases
- Fungal Proteins
- Genetic Techniques
- Mutation
- Plasmids
- Rad52 DNA Repair and Recombination Protein