A new strategy to reduce allelic bias in RNA-Seq readmapping.

Abstract excerpt

Accurate estimation of expression levels from RNA-Seq data entails precise mapping of the sequence reads to a reference genome. Because the standard reference genome contains only one allele at any given locus, reads overlapping polymorphic loci that carry a non-reference allele are at least one mismatch away from the reference and, hence, are less likely to be mapped. This bias in read mapping leads to...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alleles
• Share to Chromosome Mapping
• Share to Gene Expression Profiling