Article
Specificity of yeast KEX2 protease for variant human proalbumins is identical to the in vivo specificity of the hepatic proalbumin convertase.
The Journal of biological chemistry - 15 Dec 1990
Brennan S O, Peach R J, Bathurst I C
Abstract excerpt
Yeast KEX2 protease was examined as a potential model for a human proprotein convertase and, in all respects, mimicked the predicted properties of a proalbumin convertase. The enzyme rapidly cleaved the propeptide Arg-Gly-Val-Phe-Arg-Arg from the NH2-terminal end of proalbumin but, unlike trypsin...
Topics
- Amino Acid Sequence
- Endopeptidases
- Humans
- Hydrogen-Ion Concentration
- In Vitro Techniques
- Molecular Sequence Data
- Mutation
- Prealbumin
- Proprotein Convertases
- Protein Processing, Post-Translational
- Saccharomyces cerevisiae