Article
Mutations of the PC2 substrate binding pocket alter enzyme specificity.
The Journal of biological chemistry - 9 Sept 2005
Kacprzak Magdalena M, Than Manuel E, Juliano Luiz, Juliano Maria A, Bode Wolfram, Lindberg Iris
Abstract excerpt
By taking advantage of the recently published furin structure, whose catalytic domain shares high homology with other proprotein convertases, we designed mutations in the catalytic domain of PC2, altering residues Ser206, Thr271, Asp278, ArgGlu282, AlaSer323, Leu341, Asn365, and Ser380, which are both conserved and specific to this convertase, and substituting residues specific to PC1 and/or furin. In order to...
Topics
- Amino Acid Sequence
- Animals
- Calcium
- Catalytic Domain
- Cell Line
- Cricetinae
- Furin
- Kinetics
- Mice
- Molecular Sequence Data
- Mutation
- Proprotein Convertase 1
- Proprotein Convertase 2
- Protein Structure, Tertiary
- Sequence Alignment