Article
Enhancement of thermostability and expression level of Rasamsonia emersonii lipase in Pichia pastoris and its application in biodiesel production in a continuous flow reactor.
International journal of biological macromolecules - 1 Oct 2024
Wang Buqing, Zhou Xiaoman, Wang Yasen, Gao Yahui, Nakanishi Hideki, Fujita Morihisa, Li Zijie
Abstract excerpt
The acidic lipase from Rasamsonia emersonii named LIPR has great potential for biodiesel synthesis due to its strong methanol tolerance. Nonetheless, the limited thermostability of LIPR and low expression level in Escherichia coli remain major obstacles to its use in biodiesel synthesis. To enhan...
Topics
- Biofuels
- Lipase
- Enzyme Stability
- Bioreactors
- Saccharomycetales
- Temperature
- Mutation
- Gene Expression