Article
[Screening for mutants with thermostabe lipase A from Burkholderia sp. ZYB002].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Jun 2015
Liu Yanru, Qiu Liqing, Huang Jianzhong, Zhao Bingchun, Wang Zuozhen, Zhu Xiaolan, Gao Yuanyuan, Shu Zhengyu
Abstract excerpt
OBJECTIVE: We improved the thermostability of LipA from Burkholderia cecapia ZYB002 by protein engineering technology to expand the application of lipase LipA. METHOD: On the basis of B-factor value of lipase LipA, series of potential mutation hotspots were selected for iterative saturation mutagenesis and the corresponding small mutation gene libraries were then constructed to screen the hyperthermal variants....
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