Article
[Screening for mutants with thermostabe lipase A from Burkholderia sp. ZYB002].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Jun 2015
Liu Yanru, Qiu Liqing, Huang Jianzhong, Zhao Bingchun, Wang Zuozhen, Zhu Xiaolan, Gao Yuanyuan, Shu Zhengyu
Abstract excerpt
OBJECTIVE: We improved the thermostability of LipA from Burkholderia cecapia ZYB002 by protein engineering technology to expand the application of lipase LipA. METHOD: On the basis of B-factor value of lipase LipA, series of potential mutation hotspots were selected for iterative saturation mutag...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Burkholderia
- Enzyme Stability
- Hot Temperature
- Kinetics
- Lipase
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Protein Engineering
- Sequence Alignment
