Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Effects of nucleotide analogs at the P2X3 receptor and its mutants identify the agonist binding pouch.

Molecular pharmacology - 1 Jul 2012

Riedel Thomas, Wiese Sara, Leichsenring Anna, Illes Peter

Abstract excerpt

In this study, we investigated the effects of single alanine substitutions of amino acid residues in the supposed ATP binding site of the human P2X3 receptor on the agonistic effect of nucleotide analogs. The wild-type and mutant receptors were expressed in HEK293 cells, and the nucleotide effects were measured by means of the whole-cell patch-clamp method. Modifications in the receptor binding site changed the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenosine Triphosphate
Share to Alanine
Share to Amino Acid Substitution

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to HEK293 Cells

Share to Kinetics

Share to Nucleotides

Share to Patch-Clamp Techniques

Share to Zebrafish

Source

Identifiers

PMID: 22498660
DOI: 10.1124/mol.112.077818

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Effects of nucleotide analogs at the P2X3 receptor and its mutants identify the agonist binding pouch.PMID 22498660Read ↗

Agonist antagonist interactions at the rapidly desensitizing P2X3 receptor.Semantic proximity Read ↗

Conserved lysin and arginin residues in the extracellular loop of P2X(3) receptors are involved in agonist binding.Semantic proximity Read ↗

Participation of the Lys313-Ile333 sequence of the purinergic P2X4 receptor in agonist binding and transduction of signals to the channel gate.Semantic proximity Read ↗

Conformational flexibility of the agonist binding jaw of the human P2X3 receptor is a prerequisite for channel opening.Semantic proximity Read ↗

Molecular Structure and Regulation of P2X Receptors With a Special Emphasis on the Role of P2X2 in the Auditory System.Semantic proximity Read ↗

Identification of negative residues in the P2X3 ATP receptor ectodomain as structural determinants for desensitization and the Ca2+-sensing modulatory sites.Semantic proximity Read ↗

Functional differences between ATP-gated human and rat P2X3 receptors are caused by critical residues of the intracellular C-terminal domain.Semantic proximity Read ↗

Use-dependent inhibition of P2X3 receptors by nanomolar agonist.Semantic proximity Read ↗

ATP binding site mutagenesis reveals different subunit stoichiometry of functional P2X2/3 and P2X2/6 receptors.Semantic proximity Read ↗

A hydrophobic residue in position 15 of the rP2X3 receptor slows desensitization and reveals properties beneficial for pharmacological analysis and high-throughput screening.Semantic proximity Read ↗

Intermediate closed channel state(s) precede(s) activation in the ATP-gated P2X2 receptor.Semantic proximity Read ↗

Cryo-EM reveals the structural basis of subtype-specific, noncompetitive inhibition of the human P2X3 receptorSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.