Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Effect of 5' splice site mutations on splicing of the preceding intron.

Molecular and cellular biology - 1 Dec 1990

Talerico M, Berget S M

Abstract excerpt

Three exon constructs containing identical intron and exon sequences were mutated at the 5' splice site beginning intron 2 and assayed for the effect of the mutation on splicing of the upstream intron in vitro. Alteration of two or six bases within the 5' splice site reduced removal of intron 1 at least 20-fold, as determined by quantitation of either spliced product or released lariat RNA. The prominent product...

Topics

Adenoviruses, Human
Animals
Base Sequence
Exons
Humans
Introns
Molecular Sequence Data
Mutagenesis, Site-Directed
Phenotype
RNA Precursors
RNA Splicing
Sequence Homology, Nucleic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 2247057
DOI: 10.1128/mcb.10.12.6299-6305.1990
PMCID: PMC362905

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2247057-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Effect of 5' splice site mutations on splicing of the preceding intron.PMID 2247057Read ↗

Sequence requirements in different steps of the pre-mRNA splicing reaction: analysis by the RNA modification-exclusion technique.Semantic proximity Read ↗

Sequences within the last intron function in RNA 3'-end formation in cultured cells.Semantic proximity Read ↗

The role of exon sequences in C complex spliceosome structure.Semantic proximity Read ↗

Genetic selection for balanced retroviral splicing: novel regulation involving the second step can be mediated by transitions in the polypyrimidine tract.Semantic proximity Read ↗

Transcription of a nematode trans-spliced leader RNA requires internal elements for both initiation and 3' end-formation.Semantic proximity Read ↗

Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns.Semantic proximity Read ↗

The role of branchpoint and 3'-exon sequences in the control of balanced splicing of avian retrovirus RNA.Semantic proximity Read ↗

Elements essential for processing intronic U14 snoRNA are located at the termini of the mature snoRNA sequence and include conserved nucleotide boxes C and D.Semantic proximity Read ↗

Length suppression in histone messenger RNA 3'-end maturation: processing defects of insertion mutant premessenger RNAs can be compensated by insertions into the U7 small nuclear RNA.Semantic proximity Read ↗

Redefinition of exon 7 in the COL1A1 gene of type I collagen by an intron 8 splice-donor-site mutation in a form of osteogenesis imperfecta: influence of intron splice order on outcome of splice-site mutation.Semantic proximity Read ↗

Effect of mutations at the lariat branch acceptor site on beta-globin pre-mRNA splicing in vitro.Semantic proximity Read ↗

RNA sequences upstream of the 3' splice site repress splicing of mutantyeast ACT1 introns.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.