Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns.

Genes & development - 1 Nov 1991

Niwa M, Berget S M

Abstract excerpt

To investigate the relationship between splicing and polyadenylation during the production of vertebrate mRNAs, we examined the effect of mutation of a poly(A) site on splicing of upstream introns. Mutation of the AAUAAA polyadenylation consensus sequence inhibited in vitro splicing of an upstream intron. The magnitude of the depression depended on the magnesium concentration. Dependence of splicing on...

Topics

Adenoviridae
Consensus Sequence
Electrophoresis, Polyacrylamide Gel
Exons
Introns
Kinetics
Magnesium
Mutation
Poly A
Polymerase Chain Reaction
RNA Precursors
RNA Splicing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Simian virus 40

Source

Identifiers

PMID: 1657710
DOI: 10.1101/gad.5.11.2086

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1657710-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns.PMID 1657710Read ↗

Point mutations in AAUAAA and the poly (A) addition site: effects on the accuracy and efficiency of cleavage and polyadenylation in vitro.Semantic proximity Read ↗

Effect of RNA secondary structure on polyadenylation site selection.Semantic proximity Read ↗

Alternative polyadenylation of adeno-associated virus type 5 RNA within an internal intron is governed by the distance between the promoter and the intron and is inhibited by U1 small nuclear RNP binding to the intervening donor.Semantic proximity Read ↗

Upstream Elements Present in the 3′-Untranslated Region of Collagen Genes Influence the Processing Efficiency of Overlapping Polyadenylation SignalsSemantic proximity Read ↗

Are U4 small nuclear ribonucleoproteins involved in polyadenylation?Semantic proximity Read ↗

Effects of the multiple polyadenylation signal AAUAAA on mRNA 3'-end formation and gene expression.Semantic proximity Read ↗

Polyadenylylation signal of the mouse thymidylate synthase gene was created by insertion of an L1 repetitive element downstream of the open reading frame.Semantic proximity Read ↗

Sequence requirements in different steps of the pre-mRNA splicing reaction: analysis by the RNA modification-exclusion technique.Semantic proximity Read ↗

Different positioning elements select poly(A) sites at the 3'-end of GCN4 mRNA in the yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

Effect of 5' splice site mutations on splicing of the preceding intron.Semantic proximity Read ↗

3'-end-forming signals of yeast mRNA.Semantic proximity Read ↗

Pre-mRNA splicing is a determinant of histone H3K36 methylation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.