Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns.

Genes & development - 1 Nov 1991

Niwa M, Berget S M

Abstract excerpt

To investigate the relationship between splicing and polyadenylation during the production of vertebrate mRNAs, we examined the effect of mutation of a poly(A) site on splicing of upstream introns. Mutation of the AAUAAA polyadenylation consensus sequence inhibited in vitro splicing of an upstream intron. The magnitude of the depression depended on the magnesium concentration. Dependence of splicing on...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Consensus Sequence
Share to Electrophoresis, Polyacrylamide Gel
Share to Exons

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Introns

Share to Kinetics

Share to Mutation

Share to Polymerase Chain Reaction

Share to RNA Splicing

Source

Identifiers

PMID: 1657710
DOI: 10.1101/gad.5.11.2086

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns.PMID 1657710Read ↗

Point mutations in AAUAAA and the poly (A) addition site: effects on the accuracy and efficiency of cleavage and polyadenylation in vitro.Semantic proximity Read ↗

Effect of RNA secondary structure on polyadenylation site selection.Semantic proximity Read ↗

Alternative polyadenylation of adeno-associated virus type 5 RNA within an internal intron is governed by the distance between the promoter and the intron and is inhibited by U1 small nuclear RNP binding to the intervening donor.Semantic proximity Read ↗

Upstream Elements Present in the 3′-Untranslated Region of Collagen Genes Influence the Processing Efficiency of Overlapping Polyadenylation SignalsSemantic proximity Read ↗

Are U4 small nuclear ribonucleoproteins involved in polyadenylation?Semantic proximity Read ↗

Effects of the multiple polyadenylation signal AAUAAA on mRNA 3'-end formation and gene expression.Semantic proximity Read ↗

Polyadenylylation signal of the mouse thymidylate synthase gene was created by insertion of an L1 repetitive element downstream of the open reading frame.Semantic proximity Read ↗

Sequence requirements in different steps of the pre-mRNA splicing reaction: analysis by the RNA modification-exclusion technique.Semantic proximity Read ↗

Different positioning elements select poly(A) sites at the 3'-end of GCN4 mRNA in the yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

Effect of 5' splice site mutations on splicing of the preceding intron.Semantic proximity Read ↗

3'-end-forming signals of yeast mRNA.Semantic proximity Read ↗

A spontaneous duplication in U6 spliceosomal RNA uncouples the early and late functions of the ACAGA element in vivo.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.