Article
RNA sequences upstream of the 3' splice site repress splicing of mutantyeast ACT1 introns.
RNA (New York, N.Y.) - 1 May 1996
Kivens W, Siliciano P G
Abstract excerpt
A yeast ACT1 intron in which both the first and last intron nucleotides are mutated, the /a-c/ intron, splices 10% as well as wild type. We selected for additional cis-acting mutations that improve the splicing of /a-c/ introns and recovered small deletions upstream of the 3' splice site. For example, deletion of nucleotides -9 and -10 upstream of the 3' splice site increased the splicing activity of the /a-c/...
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