Article
Sequence requirements in different steps of the pre-mRNA splicing reaction: analysis by the RNA modification-exclusion technique.
Molecular and cellular biology - 1 Sept 1990
Lang K M, Keller W
Abstract excerpt
The stepwise assembly of splicing complexes and the subsequent splicing reaction were analyzed by the RNA modification-exclusion technique, which generates the equivalent of a complete set of point mutations in a single reaction. We found that although the sequences surrounding the 5' splice site...
Topics
- Adenoviruses, Human
- Base Sequence
- Genetic Techniques
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- RNA Precursors
- RNA Splicing
- Transcription, Genetic