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Article

Improved negative selection protocol for Plasmodium berghei in the rodent malarial model

31 Mar 2012

Abstract excerpt

An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology...

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PMID: 22463060
DOI: 10.1186/1475-2875-11-103

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