Article
An efficient strategy for gene targeting and phenotypic assessment in the Plasmodium yoelii rodent malaria model.
Molecular and biochemical parasitology - 1 Apr 2008
Mikolajczak Sebastian A, Aly Ahmed S I, Dumpit Ronald F, Vaughan Ashley M, Kappe Stefan H I
Abstract excerpt
In this report, we describe a cloning procedure for gene replacement by double homologous recombination in Plasmodium yoelii, which requires only one digestion and ligation step. This significantly shortens the time required to complete the production of the targeting vector. Furthermore, for more efficient phenotypic evaluation of the gene knockout parasites, we have also introduced a fluorescent protein...
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