Article
Conditional mutagenesis using site-specific recombination in Plasmodium berghei.
Proceedings of the National Academy of Sciences of the United States of America - 12 Oct 2004
Carvalho Teresa Gil, Thiberge Sabine, Sakamoto Hiroshi, Ménard Robert
Abstract excerpt
Reverse genetics in Plasmodium, the genus of parasites that cause malaria, still faces major limitations. Only red blood cell stages of this haploid parasite can be transfected. Consequently, the function of many essential genes in these and subsequent stages, including those encoding vaccine candidates, cannot be addressed genetically. Here, we establish conditional mutagenesis in Plasmodium by using...
Topics
- Animals
- Crosses, Genetic
- Fertilization
- Genotype
- Humans
- Mutagenesis
- Plasmids
- Plasmodium berghei
- Recombination, Genetic
- Sequence Deletion
- Tetrahydrofolate Dehydrogenase
- Transfection