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Article

Flow cytometry-assisted rapid isolation of recombinant Plasmodium berghei parasites exemplified by functional analysis of aquaglyceroporin.

International journal for parasitology - 1 Dec 2012

Kenthirapalan Sanketha, Waters Andrew P, Matuschewski Kai, Kooij Taco W A

Abstract excerpt

The most critical bottleneck in the generation of recombinant Plasmodium berghei parasites is the mandatory in vivo cloning step following successful genetic manipulation. This study describes a new technique for rapid selection of recombinant P. berghei parasites. The method is based on flow cytometry to isolate isogenic parasite lines and represents a major advance for the field, in that it will speed the...

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Identifiers

PMID: 23137753
DOI: 10.1016/j.ijpara.2012.10.006
PMCID: PMC3521960

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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