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Article

Development and application of a positive-negative selectable marker system for use in reverse genetics in Plasmodium.

Nucleic acids research - 14 Mar 2006

Braks Joanna A M, Franke-Fayard Blandine, Kroeze Hans, Janse Chris J, Waters Andrew P

Abstract excerpt

A limitation of transfection of malaria parasites is the availability of only a low number of positive selectable markers for selection of transformed mutants. This is exacerbated for the rodent parasite Plasmodium berghei as selection of mutants is performed in vivo in laboratory rodents. We here report the development and application of a negative selection system based upon transgenic expression of a...

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PMID: 16537837
DOI: 10.1093/nar/gnj033
PMCID: PMC1401515

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