Article
Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron.
PloS one - 1 Jan 2012
Seki Takahiro, Yoshino Ken-ich, Tanaka Shigeru, Dohi Eisuke, Onji Tomoya, Yamamoto Kazuhiro, Hide Izumi, Paulson Henry L, Saito Naoaki, Sakai Norio
Abstract excerpt
BACKGROUND: Chaperone-mediated autophagy (CMA) is a selective autophagy-lysosome protein degradation pathway. The role of CMA in normal neuronal functions and in neural disease pathogenesis remains unclear, in part because there is no available method to monitor CMA activity at the single-cell level. METHODOLOGY/PRINCIPAL FINDINGS: We sought to establish a single-cell monitoring method by visualizing...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.