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Article

A Fixable Fluorescence-Quenched Substrate for Quantitation of Lysosomal Glucocerebrosidase Activity in Both Live and Fixed Cells.

Angewandte Chemie (International ed. in English) - 2 Oct 2023

Zhu Sha, Deen Matthew C, Zhu Yanping, Gilormini Pierre-André, Chen Xi, Davis Oliver B, Chin Marcus Y, Henry Anastasia G, Vocadlo David J

Abstract excerpt

Fluorogenic substrates are emerging tools that enable studying enzymatic processes within their native cellular environments. However, fluorogenic substrates that function within live cells are generally incompatible with cellular fixation, preventing their tandem application with fundamental cell biology methods such as immunocytochemistry. Here we report a simple approach to enable the chemical fixation of a...

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PMID: 37582679
DOI: 10.1002/anie.202309306

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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